CV
Education
- B.S. in College of animal science and Technology, Hebei Normal University of Science&Technology，2013-2017
- M.S. in College of animal science and Technology, Northwest A&F university, (Advisor: Dr. Wei Zehui and Dr. Sun Xiuzhu), 2017-2020
- Ph.D in Biological Science, NARA Institute of Science and Technology, (Advisor: Dr. Katsutomo Okamura) 2021-2024 (expected)
Work experience
- 2020.08-2021.03: Research Assistant
- Single cell Typing platform, Institute of Neuroscience, Chinese Academy of sciences (Advisor: )
- Duties included:
a) Bulk sequencing, 10 × genomics and Smart-seq single cell transcriptomic sequencing;
b) Verify the markers of specific cell subtypes via the RNA-scope approach; - Supervisor: Dr. Liu Zhiyong
Skills
- Bioinformatics Analysis
- PAR-CLIPseq; mRNAseq; smRNA-seq etc (using Linux shell, R, Python etc.).
- Molecule and Biochemical experiments
- DNA and RNA extraction;
- Plasmid Construction for Gene Overexpression, Knock-out, Truncation or Point Mutation;
- RT-qPCR;
- Western Blot;
- PAR-CLIP;
- Immunofluorescence and Co-Immunoprecipitation;
- Ribosome Profiling and Polysome Profiling;
- Single-cell RNAseq (including single cell suspension sample preparation, single cell sort, construction of cDNA library etc.);
- In situ hybridization and RNAscope.
- Cell Culture Technique
- Cell Culture (including granulosa cell, HEK293T, HCT116, S2R+, 3T3-L1, etc.);
- Cell proliferation and apoptosis detection;
- Flow cytometry assay
- Animal experiments
- Mouse and Rabbit Dissection;
- Mouse Tumor Implantation
Publications
Ze Li; Junyi Jiang; Xiaohua Yi; Guoyan Wang; Shuhui Wang; Xiuzhu Sun*. MicroRNA-18b is involved in rabbit granulosa cell function and granulosa cell apoptosis by targeting ESR1. Reprod Fertil Dev. 2021 Mar;33(5):363-371. DOI: 10.1071/RD20237
Ze Li; Mingli Wu; Haidong Zhao; Lujie Fan; Yu Zhang; Tingting Yuan; Shuai He; Pengfei Wang; Yunhai Zhang; Xiuzhu Sun; Shuhui Wang. The PLAG1 mRNA expression analysis among genetic variants and relevance to growth traits in Chinese cattle. [J], Animal Biotechnology. June 2019. DOI: 10.1080/10495398.2019.1632207
Ze Li; Junyi Jiang; Guoyan Wang; Xiaohua Yi; Shirong Liu; Shuai He; Xiuzhu Sun; Shuhui Wang*. Four Novel Insertion/Deletion Variants of the PPP2R2B Gene and their Effects on Growth Traits in Chinese cattle. [J], Animal Biotechnology. (Submitted)
Ze Li; Junyi Jiang; Shuhui Wang; Xiuzhu Sun; Zehui Wei. Rabbit microRNA-18b bioinformatics analysis and its targeting verification with CDK19[J]. Journal of Domestic Animal Ecology. 2020,41(09):24-29. DOI: 10.3969/j.issn.1673-1182.2020.09.005 (Chinese)
Guoyan Wang; Junyi Jiang; Ze Li; Lujie Fan; Xiuzhu Sun*; Shuhui Wang. Research Progress on Timed Artificial Insemination Procedure in Beef Cattle. [J], Chinese Journal of Animal Science. 2019. DOI:10.19556/j.0258-7033.2019-03-001(Chinese)
- Baoxia Ma; Wenlu Shen; Xu Wang; Ze Li; Kun Xu*.Application of gene-edited animal models in human disease research[J].Chinese journal of biotechnology,2020,36(05):849- 860.(Chinese)
- Lujie Fan, Mingle Dou, Xiaoyu Wang, Ze Li, Xine Shi, Xiao Li. The relationship between anti-oxidation ability of muscles and meat quality[J].Acta Zoonutrimenta Sinica.2018,30 (05):1676-1680.(Chinese)
Patents
Li Ze; Fan Lujie; Wang Guoyan; Jiang Junyi; Wang Shuhui; Sun Xiuzhu. Detection and application of 19-bp deletion polymorphism of PLAG1 gene in Chinese cattle.
Li Ze; Fan Lujie; Wang Guoyan; Jiang Junyi; Wang Shuhui; Sun Xiuzhu. Detection method and application of four repeated deletion polymorphisms of PPP2R2B gene in Chinese yellow cattle
Talks
- 2023.10.11-10.14: EMBO | EMBL Symposium | The non-coding genome, in Heidelberg, Germany EMBL
- Title: Transcriptome-wide identification of substrates of the Drosophila RNA export factor Exportin5
Teaching
Teaching Assistant @ NAIST
Teaching Assistant @ NWAFU
Honors & Awards
- National Scholarship (2015);
- Outstanding Student Leader (2016);
- First Prize of Master’s Scholarship(2018);
- Outstanding Graduate Student(2019),
- Chinese government scholarships(2022.04-2024.04) etc.